Pumpkin (Cucurbita moschata) is ranked as one of the least economic important vegetable crops in Sudan despite having high nutritive values and great industrial uses. It is grown in small holdings along rivers banks. The crop is threatened by damage caused by powdery mildew, Zucchini yellow mosaic virus (Sid Ahmed et al., 2003) and fruit flies (Gesmalla, 2000). The main objective of this study is to obtain natural sources of resistance to powdery mildew, ZYMV and/or fruit flies infestation among the indigenous pumpkin germplasm.