Transformation of Cucumber with Agrobacterium rhizogenes

Cucurbit Genetics Cooperative Report 12:35-36 (article 14) 1989 F. van der Mark, J.H.W. Bergervoet and J.B.M. Custers Institute for Horticultural Plant Breeding (IVT), P.O. Box 16, 6700 AA Wageningen, The Netherlands Agrobacterium rhizogenes includes hairy root disease in many dicotyledonous plants. The root including ability is conferred to plant cells by bacterial genes (T-DNA) which […]

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Improvements of in vitro Growth of Cucumber

Cucurbit Genetics Cooperative Report 12:20-22 (article 9) 1989 J.B.M. Custers and E.C.P. Verstappen Institute for Horticultural Plant Breeding (IVT), P.O. Box 16, 6700 AA Wageningen, The Netherlands In general, in vitro culture of cucumber (Cucumis sativus L.) meets with several problems. In regeneration studies, adventitious buds as well as somatic embryos develop poorly into plants, […]

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Development of Callus and Somatic Embryos from Zygotic Embryos of Cucumber (Cucumis sativus L.)

Cucurbit Genetics Cooperative Report 11:1-2 (article 1) 1988 J.B.M. Custers, J.E.M. van Deelen and J.H.W. Bergervoet Institute for Horticultural Plant Breeding (OVT), P.O. Box 16, 6700 AA Wageningen, The Netherlands In the cross Cucumis sativus x C, melo seeded fruits often develop, but the embryos cease growth at the globular-0shaped stage (2). Embryo rescue procedures, […]

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Embryo Size in Cucumis sativus x C. melo as Affected by Irradiation of the Pollen and Genotype of the Female Parent

Cucurbit Genetics Cooperative Report 7:94-95 (article 42) 1984 Custers, J.B.M. and J.H.W. Bergervoet Institute for Horticultural Plant Breeding, P.O. Box 16, 6700 AA Wageningen, The Netherlands The cross Cucumis sativus x C. melo so far failed because the embryos cease their development at the globular- shaped stage (2). Embryo culture procedures suitable for non- hybrid […]

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Mentor Pollen in an Interspecific Cross in Cucumis: Effects of Irradiation Dose and of Order of Application of the Two Types of Pollen

Cucurbit Genetics Cooperative Report 6:94-95 (article 48) 1983 J. B. M. Custers and A. P. M. den Nijs Institute for Horticultural Plant Breeding, P. O. Box 16, 6700 AA Wageningen, The Netherlands In recent years we regularly used irradiated mentor pollen (IMP) as a pollination aid in interspecific hybridization in Cucumis. When the control cross […]

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In vitro Culture of Embryos of Cucumis zeyheri Sond. (2n=24)

Cucurbit Genetics Cooperative Report 5:54-56 (article 26) 1982 J.B.M. Custers Institute for Horticultural Plant Breeding, P.O., Box 15, Wageningen, The Netherlands A. Khatun Bangladesh Jute Research Institute, Dacca-17, Bangladesh It has been shown previously that embryos from the cross Cucumis zeyheri* (Gene Bank no. 0181) x C. Metuliferus (Gbn 1734) stopped growth during cotyledon development and seeds with such […]

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Seedling Death in Interspecific Crosses with Cucumis africanus L.f.

Cucurbit Genetics Cooperative Report 4:58-60 (article 30) 1981 A. P. M. den Nijs, D. L. Visser and J. B. M. Custers Institute for Horticultural Plant Breeding, P. O. Box 16, Wageningen, The Netherlands Three interspecific crosses with Cucumis africanus L.f. out of our crossability analysis of African Cucumis species (4) set seed, most of which […]

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Reciprocal Crosses Between Cucumis africanus L.f. and C. metuliferus Naud. III. Effects of Pollination Aids, Physiological Condition and Genetic Constitution of the Maternal Parent on Crossability

Cucurbit Genetics Cooperative Report 4:50-53 (article 27) 1981 J. B. M. Custers, A. P. M. den Nijs and A. W. Riepma Institute for Horticultural Plant Breeding, P. O. Box 16, Wageningen, The Netherlands The positive effect of mentor pollen and amino-ethoxy-vinyl-glycine (AVG) on the success of reciprocal crosses between Cucumis africanus L.f. and C. metuliferus […]

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Heart Shape Stage Embryos of Cucumis Species More Successful in Embryo Culture than Advanced Stage Embryos

Cucurbit Genetics Cooperative Report 4:48-49 (article 26) 1981 J. B. M. Custers Institute for Horticultural Plant Breeding, P. O. Box 16, Wageningen, The Netherlands Experience with general prerequisites of the artificial culture of vital embryos from self pollinated plants of Cucumis species is likely to be helpful to the culture of abortive embryos from interspecific […]

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In vitro adventitious bud formation on seedling and embryo explants of Cucumis sativus L.

Cucurbit Genetics Cooperative Report 3:2-4 (article 1) 1980 J.B.M. Custers and J.H.W. Bergervoet Institute for Horticultural Plant Breeding, Wageningen, The Netherlands We have previously reported on the effects of illumination, explant position, and explant polarity on adventitious bud formation of seedling explants of Cucumis sativus cv. ‘Hokus’ (1). This paper reports on experiments on explant length, seedling […]

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