Attempt at Localization of male sterile-4, cut leaf and virescent Mutants in Muskmelon

Cucurbit Genetics Cooperative Report 9:64-67 (article 19) 1986

M. Pitrat, C. Ferriere and M. Ricard
INRA, Station d’Amelioration des Plantes Maraicheres, 84140 Montfavet, France

We have tried to find some linkages between male sterile-4 (ms-4) supplied by P. LOZANOV (Bulgaria) (2), cut leaf (cl) supplied by I. VELICH (Hungary) (5) or virescent (v) supplied by P.E. NUGENT (South Carolina, USA) (1) with other genes belonging to linkage groups already described (3, 4).

Male sterile-4 (ms-4) was found independent (Table 1) from genes belonging to linkage groups 1 to 7. Cut leaf (cl) was found independent (Table 2) from genes belonging to groups 1 to 7 and from ms-4. Virescent (v) has already been found independent from groups 2, 3, and 6 (4). We found it independent from genes belonging to groups 1, 4, and 7 and from ms-4 and cl (Table 3). It must be still tested with FOM-1 or Prv (synonym Wmv) from group 5.

Table 1. Segregation data observed in F2 progenies between male sterile -4 (ms-4) and si-1, Vat, gl, r, Zym, Fom-1, yg and nsv.

Linkage group	Genes	[Male fertile] ms-4+ /-	[Male sterile] ms-4/ms-4	chi square Value	(9:3:3:1) Probability
1	si-1+/- si-1/si-1	121	31	4.516	21%
		41	7
2	Vat/- Vat+/Vat+	175	61	3.030	39%
		55	12
3	gl+/- gl/gl	244	75	1.171	76%
		71	26
3	r+/- r/r	125	43	2.008	57%
		33	18
4	Zym/- Zym+/Zyn+	111	43	6.213	11%
		27	18
5	Fom-1/- Fom-1+/Fom-1+	154	57	7.719	5%
		38	25
6	yg+/- yg/yg	117	32	1.333	72%
		40	11
7	nsv+/- nsv/nsv	226	83	5.751	12%
		77	14

Table 2. Segregation data observed in F2 progenies between cut leaf (cl) mutant and si-1, Fn, Pa, Zym, Fom-1, Fom-2, ms-2, and ms-4.

Linkage group	Genes	[normal] cl+/-	[cut leaf] cl/cl	chi-square Value	(9:3:3:1) Probability
1	si-1+/- si-1/si-1	164	50	5.446	14%
		58	28
2	Fn/- Fn+/Fn+	100	39	5.796	12%
		50	11
3	Pa/Pa+ Pa+/Pa+	114	36	0.602z	90%
		59	22
4	Zym/- Zym+/Zym+	137	52	0.983	81%
		50	14
5	Fom-2/- Fom-2+/Fom-2+	110	38	0.249	97%
		38	14
6	Fom-2/- Fom-2+/Fom-2+	227	72	4.466	22%
		66	34
6	ms-2+/- ms-2/ms-2	107	37	0.236	97%
		39	12
7	nsv+/- nsv/nsv	165	52	3.218	36%
		57	26
	ms-4+/-	109	44	2.254	47%
	ms-4/ms-4	32	15

^z tested for 6:3:2:1 segregation because Pa/Pa is lethal.

Table 3. Segregation data observed among the normal green plants in F2 progenies segregating for virescent (v) and si-1, Zym, Fom-2, nsv, ms-4, and cl.

Linkage group	Genotypes	segregation among normal (v+/-) plants			chi-square (3 : 1)
					Value	Probability
1	si-1+/ : si-1/si-1	121	:	34	0.776	38%
4	Zym/- : Zym+ /Zym+	125	:	39	0.130	72%
6	Fom-2/- : Fom-2+/Fom-2+	229	:	70	0.402	53%
7	nsv+/- : nsv/nsv	141	:	47	0.428	93%
	ms-4+/- : ms-4/ms-4	127	:	34	1.294	26%
	cl+/- : cl/cl	222	:	80	0.358	55%

Literature Cited

1. Hoffman, J.C. and P.E. Nugent, 1973. Inheritance of a virescent mutant of muskmelon. J. Hered. 64:311-312.
2. Locanov, P., 1983. Selekcija na mazkosterilini roditelski komponenti za ulesnjavana na proizvodstvoto na hibridni semena ot papesi. Dokl. na parva nauchna ikonferencija po genetika i selekapam Razgrad.
3. Pitrat, M., 1984. Linkage studies in muskmelon. Cucurbit Genetics Coop. Rept. 7:51-53.
4. Pitrat, M., 1985. Genetic linkages in muskmelon. Cucurbit Genetics Coop. Rept. 8:50-54.
5. Velich, I. and I. Fulop, 1970. A new muskmelon type of cut leaf character. Zoldsegtermesztes 4:107-112.