Bacterial fruit blotch (BFB) caused by Acidovoraxcitrulli is a major seed-borne disease that affects watermelon (Citrullus lanatus) seedling and fruit production around the world (5,9). A. citrulli can infect all growth stages of the watermelon plant: seeds, seedlings, foliage, flowers, and fruit(5). The scant research on fruit resistance in favor of foliar resistance has three probable explanations: 1- large-scale screening at the fruit stage is resource-intensive (4); 2- foliar resistance may correlate with fruit resistance and, even with susceptible fruit, it may be sufficient for disease control (4);3- fruit inoculation methods have not been well established.As far as investigated, there have not been any significant evaluations of fruit inoculation methods. An optimized fruit resistance assay would allow researchers to efficiently conduct large-scale screenings, and breed for fruit resistance. Here we report the evaluation of two watermelon fruit inoculation methods under field conditions.